High Resolution Studies of the Afa/Dr Adhesin Drae and its Interaction with Chloramphenicol

An Atomic Resolution Model for Assmebly, Architecture,and Function of the Dr Adhesins

RGHHHHHHGSFTPSGTTGTTKLTVTEKCQVRVGDLTVAKTRGQLTDAAPIGPVTVQALGCDARQVALKADTDNFEQGKFF
LISDNNRDKLYVNIRPTDNSAWTTDNGVFYKNDVGSWGGIIGIYVDGQQTNTPPGNYTLTLTGGYWAK

2.0 M AMMONIUM SULPHATE, 0.1M TRIS-HCL, PH 7.0

COMPLEX OF E. COLI DRAE ADHESIN WITH CHLORAMPHENICOL

SEMET AFAE-3 ADHESIN FROM ESCHERICHIA COLI

SHEET
THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN
ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW,
TWO SHEETS ARE DEFINED.

MAXIMUM LIKELIHOOD BUSTER-TNT REFINEMENT

THE MONOMERS ARE ARRANGED IN TWO TRIMERS
ADE AND BCF, EACHLINKED INTERNALLY BY 3
INTERMOLECULAR DISULPHIDE BONDS: CYSA 19 - CYS D
51 CYS D 19 - CYS E 51 CYS E 19 - CYS A
51 CYSB 19 - CYS C 51 CYS C 19 - CYS F 51
CYS F 19 - CYS B 51

ADHESIN, PROTEIN BINDING FIMBRIAL ADHESIN, UPEC, DAEC, PROTEIN BINDING