Solution structure of VBS2 fragment of talin
A vinculin binding domain from the talin rod unfolds to form a complex with the vinculin head.
GSHMQAATEDGQLLRGVGAAATAVTQALNELLQHVKAHATGAGPAGRYDQATDTILTVTENIFSSMGDAGEMVRQARILA QATSDLVNAIKADAEGESDLENSRKLLSAAKILADATAKMVEAAKGAAAHPDSEEQQQR
The structure was determined using triple-resonance NMR spectroscopy
The structure is based on 3018 NOE distance restraints and 176 dihedral talos restraints
distance geometry, tortion angle dynamics, simmulated annealing
1.5mM talin 755-889 U-15N, 13C; 20mM phosphate buffer, 20mM NaCl, 0.02% NaN3
1.5mM talin 755-889 U-15N; 20mM phosphate buffer, 20mM NaCl, 0.02% NaN3
1.5mM talin 755-889; 20mM phosphate buffer, 20mM NaCl, 0.02% NaN3
4-helix bundle, left-handed, STRUCTURAL PROTEIN
QAATEDGQLLRGVGAAATAVTQALNELLQHVKAHATGAGPAGRYDQATDTILTVTENIFSSMGDAGEMVRQARILAQATS DLVNAIKADAEGESDLENSRKLLSAAKILADATAKMVEAAKGAAAHPDSEEQQQR