Structure of a Chordin-like Cysteine-rich Repeat (VWC module) from Collagen IIA
Solution structure and dynamics of a prototypical chordin-like cysteine-rich repeat (von Willebrand Factor type C module) from collagen IIA
Identification and removal of O-linked and non-covalently linked sugars from recombinant protein produced using Pichia pastoris
collagen II, alpha-1 polypeptide; cartilage collagen; chondrocalcin, included; COL11A3, formerly
Sequential assignments were made using triple resonance techniques. Disulfide bond connectivity was determined using NOESY data acquired at different temperatures.
semi-automated assignment and torsion angle dynamics
0.8mM U-15N exon2; 50mM phosphate buffer; 0.02% NaN3; 90% H2O, 10% D2O
0.8mM U-15N exon2; 50mM phosphate buffer; 0.02% NaN3; 99.9% D2O
0.8mM U-15N,13C exon2; 50mM phosphate buffer; 0.02% NaN3; 90% H2O, 10% D2O
5 disulfide bonds, two sub-domain architecture, beta-sheet, STRUCTURAL PROTEIN