The Structure of (3R)-hydroxyacyl-ACP dehydratase (FabZ)

The Structure of (3R)-hydroxyacyl-acyl carrier protein dehydratase (FabZ) from Pseudomonas aeruginosa

(3R)-hydroxymyristoyl-[acyl carrier protein] dehydratase

MGSSHHHHHHSSGLVPRGSH(MSE)(MSE)DINEIREYLPHRYPFLLVDRVVELDIEGKRIRAYKNVSINEPFFNGHFPE
HPI(MSE)PGVLIIEA(MSE)AQAAGILGFK(MSE)LDVKPADGTLYYFVGSDKLRFRQPVLPGDQLQLHAKFISVKRSI
WKFDCHATVDDKPVCSAEIICAERKLGS

MGSSHHHHHHSSGLVPRGSHMMDINEIREYLPHRYPFLLVDRVVELDIEGKRIRAYKNVSINEPFFNGHFPEHPIMPGVL
IIEAMAQAAGILGFKMLDVKPADGTLYYFVGSDKLRFRQPVLPGDQLQLHAKFISVKRSIWKFDCHATVDDKPVCSAEII
CAERKLGS

Friedel pairs were used to solve this structure.

Ammonium Sulphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

(3R)-hydroxymyristoyl-[acyl carrier protein] dehydratase (E.C.4.2.1.-)

The six chains in the asymmetric unit comprise the biological hexamer

dehydratase; fatty acid biosynthesis; hot dog fold, LYASE

MMDINEIREYLPHRYPFLLVDRVVELDIEGKRIRAYKNVSINEPFFNGHFPEHPIMPGVLIIEAMAQAAGILGFKMLDVK
PADGTLYYFVGSDKLRFRQPVLPGDQLQLHAKFISVKRSIWKFDCHATVDDKPVCSAEIICAERKL