ZZ Domain of CBP- a Novel Fold for a Protein Interaction Module
ZZ domain of CBP: an unusual zinc finger fold in a protein interaction module
ZZ domain of murine CBP (residues 1700-1751)
This structure was determined using standard 3D homonuclear techniques, and by Cd113 NMR of a cadmium substituted sample to confirm metal coordinating ligands and coordinating atoms.
The calculated structures are based on a total of 1846 NOEs, and include duplicate NOEs. 441 intraresidue, 328 sequential, 157 medium range, 386 long range, 534 ambigous, 5 defined hydrogen bonds from H/D exchange and 29 Phi and 29 Psi constraints and 22 Chi1 constraints.
torsion angle dynamics and simulated annealing
2.3 mM solutions in degassed 10 mM Tris-d11 pH 6.8, 200 M ZnCl2, 10 mM DTT-d10, 0.05% w/v sodium azide, 94 % H2O, 6 % D2O