Solution Structure of the Apo and Copper(I)-Loaded Human Metallochaperone HAH1.
the structures were based on a total of 1219 meaningful distance constraints, 99 dihedral angle restraints.
torsion angle dynamics coupled with simulated annealing followed by restrained energy minimization
1.0 mM Cu(I)HAH1 U-15N; 10 mM sodium acetate
2 mM Cu(I)HAH1 U-95% 13C,U-98% 15N; 4 mM DTT; 100 mM phosphate buffer
copper protein, copper chaperone, Menkes, Wilson, Structural Proteomics in Europe, SPINE, Structural Genomics, METAL TRANSPORT