Crystal structure of quinoline 2-Oxidoreductase from Pseudomonas Putida 86

Active site geometry and substrate recognition of the molybdenum hydroxylase quinoline 2-oxidoreductase.

quinoline 2-oxidoreductase small subunit

quinoline 2-oxidoreductase large subunit

quinoline 2-oxidoreductase medium subunit

MQAHEESQLMRISATINGKPRVFYVEPRMHLADALREVVGLTGTKIGCEQGVCGSCTILIDGAPMRSCLTLAVQAEGCSI
ETVEGLSQGEKLNALQDSFRRHHALQCGFCTAGMLATARSILAENPAPSRDEVREVMSGNLCRCTGYETIIDAITDPAVA
EAARRGEV

MMKHEVVALKKKSIGTSVLRREDTRLLTGRGRYIADLVLSGMLHVASLRSPFAHARIVSIDVADAQALPGVELVWCGADV
AELSQGIVATMQVEGFQTTIQPLLANGVTRFVGEIVAVVVASSRAIAEDAAQLIQVEYEELPAVTGIEAALEGEARANDT
LAGNVVSRTSRARDELAPIFASSAGVVRGQFSCGRVSACPMETRGAVAQYEWTTQQLILWTATQMPSFVRTMVAMFCAIP
EHLIEVRVPDVGGGFGQKAHLHPEELLVCLLSRALGRPVRWIEDRQENFLGATHAKQQRNEMGLAFDGDGRFLALENRSI
TDGGAYNNLPWTQLVESHVGNAVILGVYKVPAVSEESIAVATNKCPIGAYRGVGFTAGQIARETLIDRAARQLGLSPFEI
RRRNVVMPEDFPFTNRLGQTHREGTYLQTINLLEEMVNPEAFRQRQAEARARGKYLGLGVSVFNEVTGTGTRTLSFLGTP
TTTHDSATVRIDPTGKVTVTTSLASSGQGHETTLAQIAADVLGVPASDVVIQAGSTKNTYGFGAYASRGAVIGAGSIGRA
ASIVRERVKQLAGHLLEAASEDIVIEDGLVHVAGVPAKGMPFAEVVGAAYFADATHPPGFDATLEATATYDPSDLVLANG
GHAAIVEIDASTYATRVTDFFAVEDCGTMINPMIVEGQIRGGIAQAIGQTLLEEVIYDDFGQLVTTTLMDYLIPTTLDVP
DIRIRHLETPSPLVPGGIKGMGESAMISAPAAVVAAVNDALAHLEVVIETVPITPERIFRSIQERPMQ

MKFPAFSYRAPASLQEVIQVLADDPDARIIAGGQSLLPLLAFRLVYPSCLVDLRNVSELFEISQSAGILSVGAMVTHFRN
KTDPTVAKCVPILPKVLAHVAHQAVRNRGTLGGSLAHADAGAEMPFLMATLGATMYIASSAGVRSVSATDFMKGHYFTDL
EAGEVLVRVEIPIPALHWEFDEYARRKGDYALVMAAAGLSMQGGRCVAARIALGAVEERAHQAIRANDFLVGKVIDESTA
ATAAELATEGLEPRSDIHGSRDLRLSLAKAITQRVILKAAQGAMYAGA

Tris-HCl, ammonium sulfate, glycerol, pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K

SEQUENCE
The authors state that the electron density
is very clear for these side-chains and there
are the same differences in the corresponding
DNA sequence.

The second part of the biological assembly is generated by the two fold axis