Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain
Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain.
STRUCTURE AND NUCLEIC-ACID BINDING OF THE DROSOPHILA ARGONAUTE 2 PAZ DOMAIN
GAMAMPMIEYLERFSLKAKINNTTNLDYSRRFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFEHDGKKVT IASYFHSRNYPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQA
SEQUENCE RESIDUES 1-4 IN THE CONSTRUCT USED FOR THE STRUCTURE DETERMINATION ARE FROM THE EXPRESSION VECTOR.
STRUCTURAL RESTRAINTS WERE DERIVED FROM 13C AND 15N-EDITED AND EDITED FILTERED NOESY EXPERIMENTS
THE EXPERIMENTALLY DETERMINED DISTANCE RESTRAINTS WERE APPLIED IN A MIXED TORSION AND CARTESIA DYNAMICS SIMULATED ANNEALING PROTOCOL. THE FINAL STRUCTURE ENSEMBLE WAS REFINED IN A SHELL OF WATER MOLECULES.
0.2-1 MM 15N OR 15N,13C-LABELED PROTEIN, 0.1-2.5 MM UNLABELED DNA, 50 MM SODIUM PHOSPHATE BUFFER, 0.2 MM DTT
nucleic acid binding protein, dna, nucleic acid binding protein-dna COMPLEX
MPMIEYLERFSLKAKINNTTNLDYSRRFLEPFLRGINVVYTPPQSFQSAPRVYRVNGLSRAPASSETFEHDGKKVTIASY FHSRNYPLKFPQLHCLNVGSSIKSILLPIELCSIEEGQA