High resolution structure of a picornaviral internal cis-acting replication element
High resolution structure of a picornaviral internal cis-acting replication element(cre).
Minimal functional sequence of HRV-14 CRE
The RNA sequence was synthesized using T7 system. The sequence occurs in Human Rhinovirus type 14.
distance geometry, molecular dynamics, simulated annealing
1.4 mM RNA U-15N,13C, 10 mM phosphate buffer (pH 6.8), 10 mM KCl, 0.02 mM EDTA, 90% H2O, 10% D2O
1.8 mM RNA, 8-10 mM phosphate buffer (pH 6.8), 10 mM KCl, 0.02 mM EDTA, 90% H2O, 10% D2O
1.8 mM RNA, 8-10 mM phosphate buffer (pH 6.8), 10 mM KCl, 0.4-4 mM Mg2+, 90% H2O, 10% D2O
0.8 mM RNA, 15N,13C (only the A residues), 10 mM phosphate buffer (pH 6.8), 10 mM KCl, 0.02 mM EDTA, 100% D2O
CRE, picornavirus, rhino virus, stem loop, RNA