NMR solution structure of the U box domain from AtPUB14, an armadillo repeat containing protein from Arabidopsis thaliana
Structure and Biochemical Function of a Prototypical Arabidopsis U-box Domain
Solution structure of a U box domain from the yeast protein Prp19p
The structure was determined using triple-resonance NMR spectroscopy
structures with favorable non-bond energy, structures with the least restraint violations
3D_15N-separated TOCSY, 3D_15N-separated NOESY
3D_13C-separated TOCSY, 3D_13C-separated NOESY
the structures are based on a total of 1083 NOE-derived distance constraints and 88 dihedral angle contraints
torsion angle dynamics, simulated annealing
0.9 mM GSPEF-AtPUB14(249-321) U-15N,13C, 20 mM sodium phosphate, 0.15 M NaCl, 1 mM DTT, 10% D20
0.6 mM GSPEF-AtPUB14(249-321) U-15N,13C, 20 mM sodium phosphate, 0.15 M NaCl, 1 mM DTT, 10% D20
0.6 mM GSPEF-AtPUB14(249-321) U-15N, 20 mM sodium phosphate, 0.15 M NaCl, 1 mM DTT, 10% D20
0.4 mM GSPEF-AtPUB14(249-321) U-15N,13C, 20 mM sodium phosphate, 0.15 M NaCl, 1 mM DTT, 99.96% D20
0.5 mM GSPEF-AtPUB14(249-321) U-15N,13C, 20 mM sodium phosphate, 0.15 M NaCl, 1 mM DTT, 99.96% D20
UBIQUITIN LIGASE, E3 LIGASE, ARABIDOPSIS, U-BOX, LIGASE