Crystal structure of phosphoenolpyruvate mutase in high ionic strength

Conformational Flexibility of PEP Mutase

Phosphoenolpyruvate mutase, PEP mutase, PEP phosphomutase

MSTKVKKTTQLKQMLNSKDLEFIMEAHNGLSARIVQEAGFKGIWGSGLSVSAQLGVRDSNEASWTQVVEVLEFMSDASDV
PILLDADTGYGNFNNARRLVRKLEDRGVAGACLEDKLFPKTNSLHDGRAQPLADIEEFALKIKACKDSQTDPDFCIVARV
EAFIAGWGLDEALKRAEAYRNAGADAILMHSKKADPSDIEAFMKAWNNQGPVVIVPTKYYKTPTDHFRDMGVSMVIWANH
NLRASVSAIQQTTKQIYDDQSLVNVEDKIVSVKEIFRLQRDDELVQAEDKYLPKN

(NH4)2SO4, HEPES, MgCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 314K

BIOMOLECULE: 1
THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC
UNIT WHICH CONSISTS OF 1 CHAIN. THE BIOLOGICAL UNIT
CANNOT BE GENERATED FROM THIS CRYSTAL FORM. THE
BIOLOGICAL UNIT IS A TETRAMER, BUT THIS CRYSTAL FORM
CONTAINS ONLY DIMERS.

SEQUENCE
THE AUTHORS INFORMED THAT THEIR SEQUENCE IS CORRECT
AT THE POSITIONS WHERE IT CONFLICTS WITH THE SWISS
PROT SEQUENCE.

Tetramer. However, the high ionic strength structure is dimeric.

phosphonopyruvate, phosphonate biosynthesis pathway, ISOMERASE

MSTKVKKTTQLKQALNSKDLEFIAEAHNGLSARIVQEAGFKGIWGSGLSVSAQLGVRDSNEASWTQVVEVLEFASDASDV
PILLDADTGYGNFNNARRLVRKLEDRGVAGACLEDKLFPKTNSLHDGRAQPLADIEEFALKIKACKDSQTDPDFCIVARV
EAFIAGWGLDEALKRAEAYRNAGADAILAHSKKADPSDIEAFAKAWNNQGPVVIVPTKYYKTPTDHFRDAGVSAVIWANH
NLRASVSAIQQTTKQIYDDQSLVNVEDKIVSVKEIFRLQRDDELVQAEDKYLPKN