The Solution Structure of the Archaeglobus fulgidis protein AF2095. Northeast Structural Genomics Consortium target GR4
Northeast Structural Genomics Consortium (NESG)
Solution structure of Archaeglobus fulgidis peptidyl-tRNA hydrolase (Pth2) provides evidence for an extensive conserved family of Pth2 enzymes in archea, bacteria, and eukaryotes
1H, 13C and 15N assignments for the Archaeglobus fulgidis protein AF2095.
MTLKQVIVVRDDLKLSRGKLAVQVAHAAIIGYLKSDSSLRRKWLDEGQKKVVLKVKSLEELLGIKHKAESLGLVTGLVQD AGLTEVPPGTITAVVIGPDEERKIDKVTGNLPLLKLEHHHHHH
Northeast Structural Genomics Consortium
triple-resonance experiments for backbone/sidechain assignments
20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN3
distance geometry simulated annealing molecular dynamics
20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN3, 5% D2O pH 6.5
Schwieters, Kuszewski, Tjandra, Clore, Brunger
Zimmerman, Moseley, Kiriyevs,Kulikowski,Montelione
Beta-sheet of 4 parallel, anti-parallel beta-strands and 3 alpha-helices, Structural Genomics, PSI, Protein Structure Initiative, Northeast Structural Genomics Consortium, NESG, Unknown function
MTLKQVIVVRDDLKLSRGKLAVQVAHAAIIGYLKSDSSLRRKWLDEGQKKVVLKVKSLEELLGIKHKAESLGLVTGLVQD AGLTEVPPGTITAVVIGPDEERKIDKVTGNLPLLK