Crystal structure of Ustilago sphaerogena ribonuclease U2 at 1.8 A resolution.
CDIPQSTNCGGNVYSNDDINTAIQGALDDVANGDRPDNYPHQYY(IAS)EASEDITLCCGSGPWSEFPLVYNGPYYSSRD NYVSPGPDRVIYQTNTGEFCATVTHTGAASYDGFTQCS
CDIPQSTNCGGNVYSNDDINTAIQGALDDVANGDRPDNYPHQYYDEASEDITLCCGSGPWSEFPLVYNGPYYSSRDNYVS PGPDRVIYQTNTGEFCATVTHTGAASYDGFTQCS
CRYSTALS WERE PREPARED BY HANGING-DROP VAPOUR-DIFFUSION METHOD AT 293 KELVIN FROM A 20 MG/ML PROTEIN SOLUTION CONTAINING 15 MG/ML 2'-DEOXY 2'-FLUORO ADENYLYL-3',5'-CYTIDINE, 0.4M AMMONIUM SULFATE, EQUILIBRATED AGAINST A RESERVOIR OF 0.9M AMMONIUM SULFATE CONTAINING 0.1M ACETATE BUFFER (PH 4.5)., vapor diffusion - hanging drop
ASP 45 IS ISOMERIZED TO L-ISOASPARTATE. A PEPTIDE BOND IS FORMED BETWEEN ASP 45 CG AND GLU 46 N.
HYDROLASE, ENDORIBONUCLEASE, BETA-ISOMERIZED ASPARTATE