STRUCTURE OF RIBONUCLEASE H PHASED AT 2 ANGSTROMS RESOLUTION BY MAD ANALYSIS OF THE SELENOMETHIONYL PROTEIN
THERE ARE THREE REGIONS OF LOW (OR NO) DENSITY, CONSISTING OF MSE 1 - LYS 3 (N TERMINUS), TYR 28 - ARG 31 (LOOP BETWEEN BETA STRAND 1 AND BETA STRAND 2), AND VAL 153 - VAL 155 (C TERMINUS). NO COORDINATES ARE PRESENT IN THIS ENTRY FOR MSE 1 AND THE THREE C-TERMINAL RESIDUES.
Structure of ribonuclease H phased at 2 A resolution by MAD analysis of the selenomethionyl protein.
Expression, Purification, and Crystallization of Natural and Selenomethionyl Recombinant Ribonuclease H from Escherichia Coli
(MSE)LKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNR(MSE)EL(MSE)AAIVALEALKEHCEVILS TDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVDLWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAA(MSE)NP TLEDTGYQVEV
MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQ WIHNWKKRGWKTADKKPVKNVDLWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV
SELENOMETHIONYL RIBONUCLEASE H (E.C.3.1.26.4)