Structure and mechanism of mRNA cap (guanine N-7) methyltransferase

Structure and mechanism of mRNA cap (Guanine-n7) methyltransferase

mRNA cap (guanine N-7) methyltransferase Ecm1

MDSSSPLKTFRKDQAMEGKKEEIREHYNSIRERGRESRQRSKTINIRNANNFIKACLIRLYTKRGDSVLDLGCGKGGDLL
KYERAGIGEYYGVDIAEVSINDARVRARNMKRRFKVFFRAQDSYGRHMDLGKEFDVISSQFSFHYAFSTSESLDIAQRNI
ARHLRPGGYFIMTVPSRDVILERYKQGRMSNDFYKIELEKMEDVPMESVREYRFTLLDSVNNCIEYFVDFTRMVDGFKRL
GLSLVERKGFIDFYEDEGRRNPELSKKMGLGCLTREESEVVGIYEVVVFRKLVPESDA

1.2M Na/K tartrate, 50mM BIS/TRIS, 20mM DTT, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 291K

HETEROGEN
7mG and ribose are disordered leaving only a GTP
modeled in the structure

methyltransferase, rna, cap, m7G, messenger rna cap, transferase