CRYSTAL STRUCTURE OF A FLUORESCENT DERIVATIVE OF RNASE A
Fluorescent Probe of Ribonuclease A Conformation
ETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSI TDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHFDASV
CSCL WAS WASHED OUT IN 80% AMMONIUM SULFATE BEFORE DATA COLLECTION.
THE FLUORESCENT LABEL AEDANS AND 99 WATER MOLECULES WERE ADDED TO THE MODEL.
REFINEMENT WAS STARTED FROM THE ISOMORPHOUS STRUCTURE OF THE SEMISYNTHETIC (1-118:111-124(D121A)).
RIBONUCLEASE A (E.C.3.1.27.5) FLUORESCENT DERIVATIVE [RNASE 1-124 (AENS-HIS-12)] FROM AMMONIUM SULFATE CRYSTAL
MALKSLVLLSLLVLVLLLVRVQPSLGKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADV QAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHFDASV