Crystal structure of human estrogenic 17beta-hydroxysteroid dehydrogenase complex with androstenedione and NADP

NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE

Cofactor hydrogen bonding onto the protein main chain is conserved in the short chain dehydrogenase/reductase family and contributes to nicotinamide orientation.

17-beta-HSD 1, Placental 17-beta-hydroxysteroid dehydrogenase, 20 alpha- hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH

ARTVVLITGCSSGIGLHLAVRLASDPSQSFKVYATLRDLKTQGRLWEAARALACPPGSLETLQLDVRDSKSVAAARERVT
EGRVDVLVCNAGLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASKF
ALEGLCESLAVLLLPFGVHLSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVF
LTALRAPKPTLRYFTTERFLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGGAGPGAEDEAGRSAVGDPELG
DPPAAPQ

PEG 4000, magnisum chloride, beta-octylglucoside, HEPES, glycerol, soaking with testosterone and NADP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystal structure of human estrogenic 17beta-hydroxysteroid dehydrogenase complex with NADP

Crystal structure of human estrogenic 17beta-hydroxysteroid dehydrogenase comlex with androstanedione and NADP

HETEROGEN

Several atoms belonging to Nicotinamide part of HETNAM 'NAP' could not be modeled and are missing from the coordinates

The second part of the biological assembly (homodimer) is generated by the two fold axis: -X, Y, -Z.

17bHSD1, androstenedione, C19-steroid, NADP binding, OXIDOREDUCTASE