CRYO-STRUCTURE OF THE PARACOCCUS DENITRIFICANS FOUR-SUBUNIT CYTOCHROME C OXIDASE IN THE COMPLETELY OXIDIZED STATE COMPLEXED WITH AN ANTIBODY FV FRAGMENT

The Cytochrome C Oxidase from Paracoccus Denitrificans Does not Change the Metal Center Ligation Upon Reduction

CYTOCHROME AA3 SUBUNIT 2, OXIDASE AA(3) SUBUNIT 2

CYTOCHROME AA3SUBUNIT 3, OXIDASE AA(3) SUBUNIT 3

GFFTRWFMSTNHKDIGILYLFTAGIVGLISVCFTVYMRMELQHPGVQYMCLEGARLIADASAECTPNGHLWNVMITYHGV
LMMFFVVIPALFGGFGNYFMPLHIGAPDMAFPRLNNLSYWMYVCGVALGVASLLAPGGNDQMGSGVGWVLYPPLSTTEAG
YSMDLAIFAVHVSGASSILGAINIITTFLNMRAPGMTLFKVPLFAWSVFITAWLILLSLPVLAGAITMLLMDRNFGTQFF
DPAGGGDPVLYQHILWFFGHPEVYIIILPGFGIISHVISTFAKKPIFGYLPMVLAMAAIGILGFVVWAHHMYTAGMSLTQ
QAYFMLATMTIAVPTGIKVFSWIATMWGGSIEFKTPMLWAFGFLFLFTVGGVTGVVLSQAPLDRVYHDTYYVVAHFHYVM
SLGAVFGIFAGVYYWIGKMSGRQYPEWAGQLHFWMMFIGSNLIFFPQHFLGRQGMPRRYIDYPVEFAYWNNISSIGAYIS
FASFLFFIGIVFYTLFAGKRVNVPNYWNEHADTLEWTLPSPPPEHTFETLPKREDWDR

QDVLGDLPVIGKPVNGGMNFQPASSPLAHDQQWLDHFVLYIITAVTIFVCLLLLICIVRFNRRANPVPARFTHNTPIEVI
WTLVPVLILVAIGAFSLPILFRSQEMPNDPDLVIKAIGHQWYWSYEYPNDGVAFDALMLEKEALADAGYSEDEYLLATDN
PVVVPVGKKVLVQVTATDVIHAWTIPAFAVKQDAVPGRIAQLWFSVDQEGVYFGQCSELCGINHAYMPIVVKAVSQEKYE
AWLAGAKEEFAA

AHVKNHDYQILPPSIWPFFGAIGAFVMLTGAVAWMKGITFFGLPVEGPWMFLIGLVGVLYVMFGWWADVVNEGETGEHTP
VVRIGLQYGFILFIMSEVMFFVAWFWAFIKNALYPMGPDSPIKDGVWPPEGIVTFDPWHLPLINTLILLLSGVAVTWAHH
AFVLEGDRKTTINGLIVAVILGVCFTGLQAYEYSHAAFGLADTVYAGAFYMATGFHGAHVIIGTIFLFVCLIRLLKGQMT
QKQHVGFEAAAWYWHFVDVVWLFLFVVIYIWGR

EVKLQESGGDLVQPGGSLKLSCAASGFTFSSYTMSWVRQTPEKRLEWVASINNGGGRTYYPDTVKGRFTISRDNAKNTLY
LQMSSLKSEDTAMYYCVRHEYYYAMDYWGQGTTVTVSSA

DIELTQTPVSLSASVGETVTITCRASENIYSYLAWYQQKQGKSPQFLVYNAKTLGEGVPSRFSGSGSGTQFSLKINSLLP
EDFGSYYCQHHYGTPPLTFGGGTKLEIK

STRUCTURE AT 2.7 ANGSTROM RESOLUTION OF THE PARACOCCUS DENITRIFICANS TWO-SUBUNIT CYTOCHROME C OXIDASE COMPLEXED WITH AN ANTIBODY FV FRAGMENT

THE SEQUENCE OF VH AND VL IS DESCRIBED IN THE FOLLOWING
REFERENCE,
OSTERMEIER C. ET AL. (1995) PROTEINS 21: 74-77.
THE NCBI REFERENCE LOCUS FOR CHAIN H IS 2914143
THE NCBI REFERENCE LOCUS FOR CHAIN L IS 2914144

THE CRYSTAL STRUCTURE SHOWS A CRYSTAL
PACKING ARRANGEMENT WHERETHERE IS CONTACT BETWEEN
CHAINS C AND H GIVING A CYCLICPACKING WITHCHAIN-
H ... (CHAIN-C...CHAIN-H) ... CHAIN-C(Y,-X,Z
) ASU: X,Y,Z (-Y,X,Z)

OXIDOREDUCTASE/IMMUNE SYSTEM, COMPLEX (OXIDOREDUCTASE-ANTIBODY), ELECTRON TRANSPORT, TRANSMEMBRANE, CYTOCHROME OXIDASE, ANTIBODY COMPLEX, OXIDOREDUCTASE-IMMUNE SYSTEM complex