HUMAN INSULIN HEXAMERS WITH CHAIN B HIS MUTATED TO TYR
Structural Consequences of the B5 Histidine --> Tyrosine Mutation in Human Insulin Characterized by X-Ray Crystallography and Conformational Analysis.
CRYSTALLISATION IN BATCH: 10 MG B5 TYR INSULIN DISSOLVED IN 2 ML 0.02M HCL. TO THIS ADDED 0.05 ML 0.15M ZINC ACETATE, 1.0 ML 0.2 M TRI-SODIUM CITRATE, 1.0 ML ACETONE. PH ADJUSTED TO 6.4-7.1 .
HUMAN INSULIN HEXAMERS WITH CHAIN B HIS MUTATED TO TYR COMPLEXED WITH RESORCINOL
HUMAN INSULIN HEXAMERS WITH CHAIN B HIS MUTATED TO TYR COMPLEXED WITH PHENOL
THE FOLLOWING SIDECHAINS HAVE BEEN ASSIGNED ZERO OCCUPANCIES DUE TO DISORDER: A4, A5, A10, B3, B4, B21, B29, C14, D3, D4 THE FOLLOWING CHAIN TERMINAL RESIDUES HAVE BEEN ASSIGNED ZERO OCCUPANCIES DUE TO DISORDER: B1-B2, B30, D30 THE FOLLOWING A CHAIN RESIDUES HAVE BEEN ASSIGNED ZERO OR HALF OCCUPANCIES DUE TO DISORDER, RESULTING FROM THE B5 HIS TO TYR MUTATION: A7, A8, A9
THE ASYMMETRIC UNIT CONTAINS AN INSULIN DIMER. THREE DIMERS AGGREGATE AROUND TWO ZINC IONS SITUATED ON A CRYSTALLOGRAPHIC3-FOLD AXIS TO FORM A HEXAMER.A MEAN DIFFERENCE IN ACCESSIBLE SURFACE AREA PER THE CHAIN IN THE COMPLEX OF 1325.5 ANGSTROM**2
HORMONE, GLUCOSE METABOLISM, DIABETES, INSULIN MUTANT