Ubiquitin Recognition by Npl4 Zinc-Fingers
Ubiquitin interactions of NZF zinc fingers.
Structure and ubiquitin interactions of the conserved zinc finger domain of Npl4.
homolog of yeast nuclear protein localization 4
(UBA52 OR UBCEP2) AND UBB AND UBC AND (UBA80 OR UBCEP1 OR RPS27A)
inter-molecular NOEs identified with half-filtered and double half-filtered NOE experiments and samples #1 and #2.
structures with the least restraint violations,structures with the lowest energy,target function
HNCACB, CBCACONH, CCONH, HCCONH,HNCO, HNCACO
structures are based on 2072 NOE-derived distance constraints, 145 dihedral angle constraints ,104 H-Bond constraints, and 14 Zinc-coordination constraints.
Torsion angle dynamics followed by simulated annealing
1mM Npl4 NZF U-15N,13C; 2mM unlabeled-Ubiquitin; 20mM phosphate buffer pH 5.5, 50mM NaCl; 90% H2O, 10%
1mM Ubiquitin U-15N,13C; 2mM unlabeled-Npl4 NZF; 20mM phosphate buffer pH 5.5, 50mM NaCl; 90% H2O, 10%
1mM Ubiquitin U-15N; 2mM unlabeled-Npl4 NZF; 20mM phosphate buffer pH 5.5, 50mM
1mM Npl4 NZF U-15N; 2mM unlabeled-Ubiquitin; 20mM phosphate buffer pH 5.5, 50mM NaCl; 90% H2O, 10%
"CRYSTALLOGRAPHY AND NMR SYSTEM (CNS): A NEW SOFTWARE SYSTEM
T.D. Goddard and D.G. Kneller, SPARKY 3,
Ubiquitin, Protein-Protein complex, Zinc-finger, Rubredoxin knuckle, NZF domain, PROTEIN BINDING