NMR structure of the Cys28His mutant (E form) of the nucleocapsid protein NCp7 of HIV-1.
Target specificity of human immunodeficiency virus type 1 NCp7 requires an intact conformation of its CCHC N-terminal zinc finger.
Structural investigation on the requirement of CCHH zinc finger type in nucleocapsid protein of human immunodeficiency virus 1.
NMR structure of the Cys28His mutant (D form) of the nucleocapsid protein NCp7 of HIV-1.
SEQUENCE The sequence here corresponds to a strain different than that found in the sequence database (GenBank accession CAB98186).
Two peptides have been chemically synthesized with and without a 15N/13C labelled Histidine residue at position 28
The structure was determined using standard 2D homonuclear experiments and 1H-15N HSQC experiments.
structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy
The structure has been calculated using 339 NOE derived distance restraints.
distance geometry, simulated annealing and molecular dynamics
2 mM His28(12-53)NCp7 90% H2O, 10% D2O with 3 equivalents of zinc
2 mM His28(12-53)NCp7 100% D2O with 3 equivalents of zinc
CCHC zinc knuckle, CCHH zinc knuckle, Viral protein