The 3D solution structure of the C-terminal region of Ku86
The 3D Solution Structure of the C-terminal Region of Ku86 (Ku86CTR)
ATP-dependent DNA helicase II, 80 kDa subunit
Lupus Ku autoantigen protein p86, Ku86, Ku80, 86 kDa subunit of Ku antigen, Thyroid- lupus autoantigen, TLAA, CTC box binding factor 85 kDa subunit, CTCBF, CTC85, Nuclear factor IV, DNA-repair protein XRCC5
GPVNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIK QLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKD
Resonance assignment for C-terminal region of Ku86
The structure was determined using triple-resonance NMR spectroscopy.
the structures are based on a total of 1959 restraints, 1863 are NOE-derived distance constraints, 174 dihedral angle restraints,96 distance restraints from hydrogen bonds, 96 residual dipolar couplings
1mM U-15N; 20mM Phosphate; 100mM NaCl; 1mM NaN3
1mM U-13C,15N; 20mM Phosphate; 100mM NaCl; 1mM NaN3
1mM U-15N; 20mM Phosphate; 100mM NaCl; 1mM NaN3; 5% n-octyl-penta(ethylene glycol):octanol 0.96:1
Ku, DNA repair, protein structure, NMR spectroscopy, DNA-PK, Ku86, Ku80, PROTEIN BINDING
VNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQL NHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKD