Structural Identification of a conserved appendage domain in the carboxyl-terminus of the COPI gamma-subunit.

Conserved Structural Motifs in Intracellular Trafficking Pathways. Structure of the gammaCOP Appendage Domain.

GSIPGLERALQQYTLEPSEKPFDLKSVPLATAPLAEQRTESTPVTAAKQPEKVAATRQEIFQEQLAAVPEFQGLGPLFKS
SPEPVALTESETEYVIRCTKHTFTDHMVFQFDCTNTLNDQTLENVTVQMEPSEAYEVLCYVPARSLPYNQPGTCYTLVAL
PKEDPTAVACTFSCVMKFTVKDCDPTTGEADDEGYEDEYVLEDLEVTIADHIQKVMKLNFEAAWDEVGDEFQKEETFTLS
TIKTLEEAVGNIVKFLGMHPCERSDKVPDNKNTHTLLLAGVFRGGHDILVRSRLLLLDTVTMQVTARSSEELPVDIVLAS
VG

100mM sodium citrate and 1.5-1.7M lithium chloride, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 18K

SEQUENCE
RESIDUES 555-603 were included in the crystalized
protein but did not show any electron
density so are presumably disordered.

platform domain, appendage domain, ear domain, ENDOCYTOSIS-EXOCYTOSIS COMPLEX

IPGLERALQQYTLEPSEKPFDLKSVPLATAPLAEQRTESTPVTAAKQPEKVAATRQEIFQEQLAAVPEFQGLGPLFKSSP
EPVALTESETEYVIRCTKHTFTDHMVFQFDCTNTLNDQTLENVTVQMEPSEAYEVLCYVPARSLPYNQPGTCYTLVALPK
EDPTAVACTFSCVMKFTVKDCDPTTGEADDEGYEDEYVLEDLEVTIADHIQKVMKLNFEAAWDEVGDEFQKEETFTLSTI
KTLEEAVGNIVKFLGMHPCERSDKVPDNKNTHTLLLAGVFRGGHDILVRSRLLLLDTVTMQVTARSSEELPVDIVLASVG