Structure of the channel-forming trans-membrane domain of Virus protein "u" (Vpu) from HIV-1
Three-dimensional structure of the channel-forming trans-membrane domain of virus protein "u" (Vpu) from HIV-1
PISEMA: Polarization Inversion Spin Exchange at the Magic Angle
This structure was constructed as a symmetric pentamer of the Vpu2-30+ trans-membrane construct based on the empirical minimization of energy upon the addition of sidechains to a backbone structure that was generated from solid-state NMR data using the program SCWRL.
Completely aligned in glass plates: 3.5 mg Vpu2-30+ U-15N, 75 mg lipid mixture (DOPC:DOPG, 9:1)
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
Bower, M.J., Cohen, F.E. & Dunbrack Jr., R.L.