Fitting of gp11 crystal structure into 3D cryo-EM reconstruction of bacteriophage T4 baseplate-tail tube complex

Three-dimensional structure of bacteriophage T4 baseplate

Structure of bacteriophage T4 gene product 11, the interface between the baseplate and short tail fibers

REFINEMENT PROTOCOL--Laplacian-filtered real space

a modified version of program SPIDER was used for the reconstruction

CTF correction of each particle using Wiener filtering

Double amber mutants of bacteriophage T4 producing baseplate and tail tube assembly only

MSLLNNKAGVISRLADFLGFRPKTGDIDVMNRQSVGSVTISQLAKGFYEPNIESAINDVHNFSIKDVGTIITNKTGVSPE
GVSQTDYWAFSGTVTDDSLPPGSPITVLVFGLPVSATTGMTAIEFVAKVRVALQEAIASFTAINSYKDHPTDGSKLEVTY
LDNQKHVLSTYSTYGITISQEIISESKPGYGTWNLLGAQTVTLDNQQTPTVFYHFERTA

the initial coordinates used for fitting

SEQUENCE
Only coordinates for CA atoms were submitted.

BIOMOLECULE: 1
THIS ENTRY CONTAINS A PORTION OF THE BIOLOGICALLY
SIGNIFICANT MULTIMER.

ASSEMBLY COMPONENTS
COM_ID: 1
NAME:GP11
IPR_ID: NULL
GO_ID: NULL
OTHER_DETAILS: TRIMER