Selenomethionine and Selenocysteine Double Labeling Strategy for Crystallographic Phasing
Structure of the cathelicidin motif of protegrin-3 precursor: structural insights into the activation mechanism of an antimicrobial protein.
Nucleoside diphosphate kinase, cytosolic
(MSE)STNKVNKERTFLAVKPDGVARGLVGEIIARYEKKGFVLVGLKQLVPTKDLAESHYAEHKERPFFGGLVSFITSGP VVA(MSE)VFEGKGVVASARL(MSE)IGVTNPLASAPGSIRGDFGVDVGRNIIUGSDSVESANREIALWFKPEELLTEVK PNPNLYE
MSTNKVNKERTFLAVKPDGVARGLVGEIIARYEKKGFVLVGLKQLVPTKDLAESHYAEHKERPFFGGLVSFITSGPVVAM VFEGKGVVASARLMIGVTNPLASAPGSIRGDFGVDVGRNIIUGSDSVESANREIALWFKPEELLTEVKPNPNLYE
PEG 6000, magnesium chloride,Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
HETEROGEN THE HETATM ENTRY SE 121 CORRESPONDS TO A SELENOCYSTEINE ADDUCT TO SEC 122 (NOT VISIBLE IN THE ELECTRON DENSITY MAP EXCEPT SELENIUM ATOM) WITH A DISELENIDE BRIDGE BETWEEN SE 121 AND SE SEC X 122
selenocysteine selenomethionine, TRANSFERASE
MSTNKVNKERTFLAVKPDGVARGLVGEIIARYEKKGFVLVGLKQLVPTKDLAESHYAEHKERPFFGGLVSFITSGPVVAM VFEGKGVVASARLMIGVTNPLASAPGSIRGDFGVDVGRNIIHGSDSVESANREIALWFKPEELLTEVKPNPNLYE