High-resolution structure of the complex of HHR23A ubiquitin-like domain and the C-terminal ubiquitin-interacting motif of proteasome subunit S5a
Structural determinants for the binding of ubiquitin-like domains to the proteasome.
26S proteasome non-ATPase regulatory subunit 4
C-terminal ubiquitin-interacting motif, PUBS2
UV excision repair protein RAD23 homolog A
26S proteasome regulatory subunit S5A, Rpn10, Multiubiquitin chain binding protein, Antisecretory factor-1, AF, ASF
High-resolution structure of the ubiquitin-like domain of HHR23A
Structure of the C-terminal ubiquitin-interacting motif of the proeasome subunit S5a in its unbound conformation
The structure was determined using triple-resonance NMR spectroscopy
The structures are based on a total of 2066 restraints, 1999 are NOE-derived distance restraints, of which 58 are intermolecular. For the S5a ubiquitin-interacting motif only the residues 270 to 301 were included in the structure calculations due to disorder of the flexible N- and C-termini.
2mM S5a peptide unlabeled, 2mM ubiquitin-like domain of HHR23A U-15N,13C
50mM sodium phosphate, 100mM sodium chloride, 5% D2O
2mM S5a peptide U-15N,13C, 2mM ubiquitin-like domain of HHR23A unlabeled