BACTERIORHODOPSIN N' INTERMEDIATE AT 1.62 A RESOLUTION

Crystallographic Structures of the M and N Intermediates of Bacteriorhodopsin: Assembly of a Hydrogen-Bonded Chain of Water Molecules between Asp-96 and the Retinal Schiff Base

QAQITGRPEWIWLALGTALMGLGTLYFLVKGMGVSDPDAKKFYAITTLAPAIAFTMYLSMLLGYGLTMVPFGGEQNPIYW
ARYADWLFTTPLLLLDLALLVDADQGTILALVGADGIMIGTGLVGALTKVYSYRFVWWAISTAAMLYILYVLFFGFTSKA
ESMRPEVASTFKVLRNVTVVLWSAYPVVWLIGSEGAGIVPLNIETLLFMVLDVSAKVGFGLILLRSRAIFGEAEAPEPSA
GDGAAATSD

MONO-OLEIN, POTASSIUM PHOSPHATE, pH 5.60, CUBIC LIPID PHASE, temperature 295K

BIOMOLECULE 1
THIS ENTRY IS MADE UP OF TWO MODELS WHICH REPRESENT
TWO CONFORMATIONS OF THE WILD-TYPE PROTEIN.
MODEL 1 IS THE N' INTERMEDIATE WITH OCCUPANCY OF 0.37 WHILE
MODEL 2 IS THE NON-ILLUMINATED BACTERIORHODOPSIN
(BR STATE) WITH OCCUPANCY OF 0.63.

ION PUMP, MEMBRANE PROTEIN, RETINAL PROTEIN, LIPIDS, PHOTORECEPTOR, HALOARCHAEA, 7-TRANSMEMBRANE, SERPENTINE, MEROHEDRAL TWINNING, PROTON TRANSPORT

QAQITGRPEWIWLALGTALMGLGTLYFLVKGMGVSDPDAKKFYAITTLVPAIAFTMYLSMLLGYGLTMVPFGGEQNPIYW
ARYADWLFTTPLLLLDLALLVDADQGTILALVGADGIMIGTGLVGALTKVYSYRFVWWAISTAAMLYILYVLFFGFTSKA
ESMRPEVASTFKVLRNVTVVLWSAYPVVWLIGSEGAGIVPLNIETLLFMVLDVSAKVGFGLILLRSRAIFGEAEAPEPSA
GDGAAATSD