The solution structure of apo CopZ from Bacillus subtilis
Solution Structure of Apo CopZ from Bacillus subtilis: Further Analysis of the Changes Associated with the Presence of Copper
Copper Trafficking: The Solution Structure of copper loaded Bacillus Subtilis Copz
The 500 MHz spectrometer is equipped with a cryo probe
structure with the lowest energy target function
A total of 1639 upper distance limits (of which 1431 meaningful), 31 dihedral phi angle constraints and 48 psi angle constraints were measured and used in the structural calculations. 5 stereospecific assignments were also obtained from the analysis of HNHB spectra and 11 through the program GLOMSA.
Simulate annealing, torsion angle dynamics
100 mM phosphate buffer pH 7.0, with 1 eq of DTT reductant and 10% D2O. All manipulations were performed under inert atmosphere.
M-X-C-X-X-C motif, beta-alpha-beta-beta-alpha-beta secondary structure, copper chaperone, CHAPERONE