Structural Insights into the Inter-domain Chaperoning of Tandem PDZ Domains in Glutamate Receptor Interacting Proteins

Tandem PDZ repeats in glutamate receptor-interacting proteins have a novel mode of PDZ domain-mediated target binding

QVVHTETTEVVLTADPVTGFGIQLQGSVFATETLSSPPLISYIEADSPAERCGVLQIGDRVMAINGIPTEDSTFEEANQL
LRDSSITSKVTLEIEFDVAESVIPSSGTFHVKLPKKHSVELGITISSPSSRKPGDPLVISDIKKGSVAHRTGTLELGDKL
LAIDNIRLDSCSMEDAVQILQQCEDLVKLKIRKDED

HNCO, HNCA, HN(CO)CA, HN(CA)CB, and HN(COCA)CB

1.5mM unlabelled PDZ45; 100mM phosphate buffer

1.5mM uniformly 15N labelled PDZ45; 100mM phosphate buffer

1.5mM uniformly 15N/13C labelled PDZ45; 100mM phosphate buffer

1.5mM 80% deuterated, uniformly 13C-/15N-labeled PDZ45; 100mM phosphate buffer

1.5mM 100% 2H, 15N-labeled PDZ45; 100mM phosphate buffer

PDZ domain, Glutamate Receptor, tandem repeats, scaffold protein, PROTEIN BINDING