NMR structure of ubiquitin-like domain of hHR23B
Binding surface mapping of intra- and interdomain interactions among hHR23B, ubiquitin, and polyubiquitin binding site 2 of S5a
UV excision repair protein RAD23 homolog B
GSHMQVTLKTLQQQTFKIDIDPEETVKALKEKIESEKGKDAFPVAGQKLIYAGKILNDDTALKEYKIDEKNFVVVMVTKP KAVST
Distance Restraints From 2D D2O-NOESY, 3D 15N-NOESY-HSQC, Angle Restaints From Intensity-modulated 15N HSQC and Talos Chemical shift analysis
CBCACONH, HNCACB, CCC-TOC-NNH, HCC-TOC-NNH, HNCO
3D_15N-separated_TOCSY, Intensity-modulated 15N HSQC
50mM sodium phosphate + 100mM sodium chloride
Cyana-Automated NOE assignment, Torsion angle dynamics; Amber-Simulated annealing, Cyana output is the initial structure
2mM UbL domain 15N,13C; 50mM Sodium Phosphate Buffer with 100mM NaCl
2mM UbL domain 15N; 50mM Sodium Phosphate Buffer with 100mM NaCl
2mM UbL domain non-labeled; 50mM Sodium Phosphate Buffer with 100mM NaCl
MQVTLKTLQQQTFKIDIDPEETVKALKEKIESEKGKDAFPVAGQKLIYAGKILNDDTALKEYKIDEKNFVVVMVTKPKAV ST