THE K136E MUTANT OF LACTOCOCCUS LACTIS DIHYDROOROTATE DEHYDROGENASE A IN COMPLEX WITH OROTATE

Lactococcus lactis dihydroorotate dehydrogenase A mutants reveal important facets of the enzymatic function.

Active Site of Dihydroorotate Dehydrogenase A from Lactococcus Lactis Investigated by Chemical Modification and Mutagenesis

The Crystals Structure of Lactococcus Lactis Dihydroorotate Dehydrogenase A Complexed with the Enzyme Reaction Product Throws Light on its Enzymatic Function

The Crystals Structure of the Flavin Containing Enzyme Dihydroorotate Dehydrogenase A from Lactococcus Lactis

Purification and Characterisation of Dihydroorotate Dehydrogenase A from Lactococcus Lactis, Crystallisation and Preliminary X-Ray Diffraction Studies of the Enzyme

Dihydroorotate oxidase A, DHOdehase A, DHODase A, DHOD A

MLNTTFANAKFANPFMNASGVHCMTIEDLEELKASQAGAYITKSSTLEKREGNPLPRYVDLELGSINSMGLPNLGFDYYL
DYVLKNQKENAQEGPIFFSIAGMSAAENIAMLKKIQESDFSGITELNLSCPNVPGEPQLAYDFEATEKLLKEVFTFFTKP
LGVKLPPYFDLVHFDIMAEILNQFPLTYVNSVNSIGNGLFIDPEAESVVIKPKDGFGGIGGAYIKPTALANVRAFYTRLK
PEIQIIGTGGIETGQDAFEHLLCGATMLQIGTALHKEGPAIFDRIIKELEEIMNQKGYQSIADFHGKLKSL

PEG 6K, Sodium acetate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Native Lactococcus lactis dihydroorotate dehydrogenase A

Native Lactococcus lactis dihydroorotate dehydrogenase A complexed with orotate

K213E mutant of Lactococcus lactis dihydroorotate dehydrogenase A

R57A mutant of Lactococcus lactis dihydroorotate dehydrogenase A

N67A mutant of Lactococcus lactis dihydroorotate dehydrogenase A

P56A mutant of Lactococcus lactis dihydroorotate dehydrogenase A

K136E mutant of Lactococcus lactis dihydroorotate dehydrogenase A

1.8 A resolution structure of native Lactococcus lactis dihydroorotate dehydrogenase A

The asymmetric unit contains the biological homodimer

HOMODIMER, ALPHA-BETA BARREL, FLAVOPROTEIN, OROTATE COMPLEX, MUTANT ENZYME, OXIDOREDUCTASE

MLNTTFANAKFANPFMNASGVHCMTIEDLEELKASQAGAYITKSSTLEKREGNPLPRYVDLELGSINSMGLPNLGFDYYL
DYVLKNQKENAQEGPIFFSIAGMSAAENIAMLKKIQESDFSGITELNLSCPNVPGKPQLAYDFEATEKLLKEVFTFFTKP
LGVKLPPYFDLVHFDIMAEILNQFPLTYVNSVNSIGNGLFIDPEAESVVIKPKDGFGGIGGAYIKPTALANVRAFYTRLK
PEIQIIGTGGIETGQDAFEHLLCGATMLQIGTALHKEGPAIFDRIIKELEEIMNQKGYQSIADFHGKLKSL