The Crystal Structure of the one-iron form of the di-iron center in Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean).

Azide and Acetate Complexes plus two iron-depleted Crystal Structures of the Di-iron Enzyme delta9 Stearoyl-ACP Desaturase-Implications for Oxygen Activation and Catalytic Intermediates

Stearoyl Acyl Carrier Protein Desaturase

ASTLKSGSKEVENLKKPFMPPREVHVQVTHSMPPQKIEIFKSLDNWAEENILVHLKPVEKCWQPQDFLPDPASDGFDEQV
RELRERAKEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRV
DMRQIEKTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNTARQAKEHGDIKLAQICGTIAADEKRHETAYTKIV
EKLFEIDPDGTVLAFADMMRKKISMPAHLMYDGRDDNLFDHFSAVAQRLGVYTAKDYADILEFLVGRWKVDKLTGLSAEG
QKAQDYVCRLPPRIRRLEERAQGRAKEAPTMPFSWIFDRQVKL

8-12% Peg8000, 50mM Potassium Dihydrogen Phosphate and 20% Glycerol as cryoprotectant, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

The Crystal Structure of Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean).

1OQ4 contains the same protein complexed with FE and AZI

1OQ7 contains the same protein complexed with strontium ion

1OQB contains the same protein complexed with FE

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

The enzyme is dimeric and the unit cell contains three of these dimers giving six protein chains

di-iron enzyme, four-helix bundle, fatty acid biosynthesis, electron transfer, OXIDOREDUCTASE