The crystal structure of the iron free (Apo-)form of Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean).

Azide and Acetate Complexes plus two iron-depleted Crystal Structures of the Di-iron Enzyme delta9 Stearoyl-ACP Desaturase-Implications for Oxygen Activation and Catalytic Intermediates

Stearoyl Acyl Carrier Protein Desaturase

ASTLKSGSKEVENLKKPFMPPREVHVQVTHSMPPQKIEIFKSLDNWAEENILVHLKPVEKCWQPQDFLPDPASDGFDEQV
RELRERAKEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRV
DMRQIEKTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNTARQAKEHGDIKLAQICGTIAADEKRHETAYTKIV
EKLFEIDPDGTVLAFADMMRKKISMPAHLMYDGRDDNLFDHFSAVAQRLGVYTAKDYADILEFLVGRWKVDKLTGLSAEG
QKAQDYVCRLPPRIRRLEERAQGRAKEAPTMPFSWIFDRQVKL

8-10% Peg6000, 60mM Strontium Chloride, 0.1M ADA buffer, 15% Glycerol, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K

The Crystal Structure of Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean).

1OQ4 contains the same protein complexed with FE and AZI

1OQ9 contains the same protein complexed with FE and ACT

1OQB contains the same protein complexed with FE

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

The enzyme is dimeric and the unit cell contains three of these dimers giving six protein chains

di-iron enzyme, four-helix bundle, fatty acid biosynthesis, electron transfer, OXIDOREDUCTASE