OMPR DNA-BINDING DOMAIN, ESCHERICHIA COLI

The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor.

APSQEEAVIAFGKFKLNLGTREMFREDEPMPLTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLR
RMVEEDPAHPRYIQTVWGLGYVFVPDGSKA

PROTEIN WAS CRYSTALLIZED FROM 3% POLYETHYLENEGLYCOL-MONOMETHYLETHER (PMME) 5000, 17.5% ETHYLENEGLYCOL, 2.5% MPD, 30 MM MES, PH 6.5; THEN SOAKED IN 10% PMME 5000, 20% ETHYLENEGLYCOL, 10% PEG 200, 200 MM AMMONIUM SULFATE, 30 MM MES, PH 6.5

TRANSCRIPTION REGULATION, RESPONSE REGULATOR, WINGED HELIX, OSMOREGULATION

MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLLTRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPII
MVTAKGEEVDRIVGLEIGADDYIPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAHPRYIQTVWGLGYVFVPDGSKA

ALPHA LOOP, SITE OF INTERACTION WITH RNA POLYMERASE ALPHA SUBUNIT.