Mutations in the T1.5 loop of pectate lyase A

Effect of mutations in the T1.5 loop of pectate lyase A from Erwinia chrysanthemi EC16.

AELVSDKALESAPTVGWASQNGFTTGGAAATSDNIYIVTNISEFTSALSAGAEAKIIQIKGTIDISGGTPYTDFADQKAR
SQINIPANTTVIGLGTDAKFINGSLIIDGTDGTNNVIIRNVYIQTPIDVEPHYEKGDGWNAEWDAMNITNGAHHVWIDHV
TISDGNFTDDMYTTKDGETYVQHDGALDIKRGSDYVTISNSLIDQHDKTMLIGHSDSNGSQDKGKLHVTLFNNVFNRVTE
RAPRVRYGSIHSFNNVFKGDAKDPVYRYQYSFGIGTSGSVLSEGNSFTIANLSASKACKVVKKFNGSIFSDNGSVLNGSA
VDLSGCGFSAYTSKIPYIYDVQPMTTELAQSITDNAGSGKL

PEG 5000 monomethyl ether (MME), 0.1M MES (2-[N-Morpholino]ethanesulfonic acid), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

PDB entry 1JTA with out residues 215-226

The biological assembly is a monomer that packs two molecules in the asymmetric unit.

AELVSDKALESAPTVGWASQNGFTTGGAAATSDNIYIVTNISEFTSALSAGAEAKIIQIKGTIDISGGTPYTDFADQKAR
SQINIPANTTVIGLGTDAKFINGSLIIDGTDGTNNVIIRNVYIQTPIDVEPHYEKGDGWNAEWDAMNITNGAHHVWIDHV
TISDGNFTDDMYTTKDGETYVQHDGALDIKRGSDYVTISNSLIDQHDKTMLIGHNDTNSAQDKGKLHVTLFNNVFNRVTE
RAPRVRYGSIHSFNNVFKGDAKDPVYRYQYSFGIGTSGSVLSEGNSFTIANLSASKACKVVKKFNGSIFSDNGSVLNGSA
VDLSGCGFSAYTSKIPYIYDVQPMTTELAQSITDNAGSGKL