Orthorhombic crystal form of an Archaeal fructose 1,6-bisphosphate aldolase

Crystal structure of an archaeal class I aldolase and the evolution of (betaalpha)8 barrel proteins.

MANLTEKFLRIFARRGKSIILAYDHGIEHGPADFMDNPDSADPEYILRLARDAGFDGVVFQRGIAEKYYDGSVPLILKLN
GKTTLYNGEPVSVANCSVEEAVSLGASAVGYTIYPGSGFEWKMFEELARIKRDAVKFDLPLVVWSYPRGGKVVNETAPEI
VAYAARIALELGADAMKIKYTGDPKTFSWAVKVAGKVPVLMSGGPKTKTEEDFLKQVEGVLEAGALGIAVGRNVWQRRDA
LKFARALAELVYGGKKLAEPLNV

9% (W/V) PEG 4000, 0.1 M NA ACETATE PH 5.0

CRYSTAL STRUCTURE OF AN ARCHAEAL FRUCTOSE 1 ,6-BISPHOSPHATE ALDOLASE

ARCHAEAL FRUCTOSE 1,6-BISPHOSPHATE ALDOLASE COVALENTLY BOUND TO THE SUBSTRATE DIHYDROXYACETONE PHOSPHATE

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY
A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "BA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY
A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "CA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY
A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "DA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY
A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "EA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY
A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "FA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY
A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "GA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY
A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "HA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY
A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "IA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY
A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

THE SHEETS PRESENTED AS "JA" IN EACH CHAIN ON SHEET RECORDS
BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY
A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS
ARE IDENTICAL.

CATALYTIC ACTIVITY: D-FRUCTOSE 1,6-BISPHOSPHATE = GLYCERONE PHOSPHATE + D-GLYCERALDEHYDE 3-PHOSPHATE.

ALDOLASE, FRUCTOSE 1, 6-BISPHOSPHATE, TIM BARREL, GLYCOLYTIC, ARCHAEAL, ORTHORHOMBIC, LYASE, SCHIFF BASE