THE CRYSTAL STRUCTURE OF MALONAMIDASE E2 FROM BRADYRHIZOBIUM JAPONICUM

Characterization of a Novel Ser-Cisser-Lys Catalytic Triad in Comparison with the Classical Ser-His-Asp Triad.

CIS PEPTIDE BOND BETWEEN GLY 130 AND SER 131

MISLADLQRRIETGELSPNAAIAQSHAAIEAREKEVHAFVRHDKSARAQASGPLRGIAVGIKDIIDTANMPTEMGSEIYR
GWQPRSDAPVVMMLKRAGATIIGKTTTTAFASRDPTATLNPHNTGHSPGGSSSGSAAAVGAGMIPLALGTQTGGSVIRPA
AYCGTAAIKPSFRMLPTVGVKCYSWALDTVGLFGARAEDLARGLLAMTGRSEFSGIVPAKAPRIGVVRQEFAGAVEPAAE
QGLQAAIKAAERAGASVQAIDLPEAVHEAWRIHPIIQDFEAHRALAWEFSEHHDEIAPMLRASLDATVGLTPKEYDEARR
IGRRGRRELGEVFEGVDVLLTYSAPGTAPAKALASTGDPRYNRLWTLMGNPCVNVPVLKVGGLPIGVQVIARFGNDAHAL
ATAWFLEDALAK

20% POLYETHYLENE GLYCOL 1000, 100 MM TRIS, PH 7.0

CRYSTAL STRUCTURE OF THE K62A MUTANT OF MALONAMIDASE E2 FROM BRADYRHIZOBIUM JAPONICUM

CRYSTAL STRUCTURE OF THE S131A MUTANT OF MALONAMIDASE E2 COMPLEXED WITH MALONAMATE FROM BRADYRHIZOBIUM JAPONICUM

CRYSTAL STRUCTURE OF THE S131A MUTANT OF MALONAMIDASE E2 COMPLEXED WITH MALONATE FROM BRADYRHIZOBIUM JAPONICUM

CRYSTAL STRUCTURE OF THE S155C MUTANT OF MALONAMIDASE E2 FROM BRADYRHIZOBIUM JAPONICUM

CRYSTAL STRUCTURE OF THE G130A MUTANT OF MALONAMIDASE E2 FROM BRADYRHIZOBIUM JAPONICUM

CRYSTAL STRUCTURE OF THE T150A MUTANT OF MALONAMIDASE E2 FROM BRADYRHIZOBIUM JAPONICUM

CRYSTAL STRUCTURE OF THE R158Q MUTANT OF MALONAMIDASE E2 FROM BRADYRHIZOBIUM JAPONICUM

CRYSTAL STRUCTURE OF THE S133A MUTANT OF MALONAMIDASE E2 COMPLEXED WITH MALONATE FROM BRADYRHIZOBIUM JAPONICUM

CRYSTAL STRUCTURE OF THE S155C MUTANT OF MALONAMIDASE E2

HYDROLYZES MALONAMATE (-OOCCH2CONH2) INTO MALONATE AND AMMONIA. INVOLVED IN THE TRANSPORT OF FIXED NITROGEN FROM BACTEROIDS TO PLANT CELLS IN SYMBIOTIC NITROGEN METABOLISM
THE RESIDUES 401-412 DIFFER FROM THAT IN THE SWISS-PROT ENTRY Q9ZIV5 DUE TO FRAME SHIFT BECAUSE OF ADDITION OF ADENINE 1202 IN THE ORIGINAL SEQUENCE.