Antibiotic binding domain of a TipA-class multidrug resistance transcriptional regulator

Structural basis for antibiotic recognition by the TipA-class of multidrug-resistance transcriptional regulators

GINLTPEEKFEVFGDFDPDQYEEEVRERWGNTDAYRQSKEKTASYTKEDWQRIQDEADELTRRFVALMDAGEPADSEGAM
DAAEDHRQGIARNHYDCGYEMHTCLGEMYVSDERFTRNIDAAKPGLAAYMRDAILANAVRHTP

Bacillus subtilis multidrug resistance regulator BmrR

Bacillus subtilis multidrug transporter regulator MTAN

standard triple and double resonance experiments as described in Grzesiek et al. (1997), Protein Science 6, 1248-1263.

standard triple and double resonance experiments

the missing residues in REMARK 465 are missing in the structure due to disorder, but had been present during the experiment.
simulated annealing with torsion angle dynamics

1.5 mM TipAS U-15N, 5mM phosphate buffer, 5mM DTT

1.5 mM TipAS U-15N,13C, 5mM phosphate buffer, 5mM DTT

0.8 mM TipAS U-15N,13C, 10 mr/ml Pf1 phages, 5mM phosphate buffer, 5mM DTT