Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP

The Structure Of The Non-Vertebrate Actin: Implications For The ATP Hydrolytic Mechanism

Actin-depolymerizing factor, ADF, Brevin, AGEL

DGEDVQALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWD
DMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVS
HTVPIYEGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEKLAYVALDFEQEMATAASSSALEKSY
ELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTAL
APSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEYDESGPSIVHRKCF

MVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDESGAAAIF
TVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGGVASGF

Lithium sulfate, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K

D. discoideum actin-gelsolin complex bound to Mg ATP at 1.93 A resolution

D. discoideum actin-gelsolin complex bound to Mg ATP at 1.80 A resolution

D. discoideum actin-gelsolin complex bound to Ca ATP

ACTIN, GELSOLIN, CYTOSKELETON ORGANIZATION, ACTIN-ASSOCIATED PROTEIN, STRUCTURAL PROTEIN