Crystal structure of Lactose synthase complex with UDP and Manganese

Crystal structure of lactose synthase reveals a large conformational change in its catalytic component, the beta-1,4-galactosyltransferase

CHAINS A AND B FORM FIRST, C AND D SECOND LACTOSE SYNTHASE COMPLEX

B4GAL-T1; BETA4GAL-T1; BETA-1,4-GALTASE 1; BETA-1,4-GALACTOSYLTRANSFERASE 1; UDP-GALACTOSE:BETA-N-ACETYLGLUCOSAMINE BETA-1,4-GALACTOSYLTRANSFERASE 1

TELTKCKVSHAIKDIDGYQGISLLEWACVLFHTSGYDTQAVVNDNGSTEYGLFQISDRFWCKSSEFPESENICGISCDKL
LDDELDDDIACAKKILAIKGIDYWKAYKPMCSEKLEQWRCEKP

ASMTGGQQMGRGSSLTACPEESPLLVGPMLIEFNIPVDLKLVEQQNPKVKLGGRYTPMDCISPHKVAIIIPFRNRQEHLK
YWLYYLHPILQRQQLDYGIYVINQAGESMFNRAKLLNVGFKEALKDYDYNCFVFSDVDLIPMNDHNTYRCFSQPRHISVA
MDKFGFSLPYVQYFGGVSALSKQQFLSINGFPNNYWGWGGEDDDIYNRLAFRGMSVSRPNAVIGKCRMIRHSRDKKNEPN
PQRFDRIAHTKETMLSDGLNSLTYMVLEVQRYPLYTKITVDIGTPS

N-terminal carries a tag containing 13 amino acids

PEG 4000, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

crystal structure of lactose synthase complex with N-acetylglucosamine

Crystal structure of lactose synthase complex with UDP

Crystal structure of lactose synthase complex with glucose

ALPHA-LACTALBUMIN/BETA-1,4-GALACTOSYLTRANSFERASE (E.C.2.4.1.22, E.C.2.4.1.90, E.C.2.4.1.38) COMPLEX

lactose synthease, UDP and MN binding, TRANSFERASE ACTIVATOR-TRANSFERASE COMPLEX