DEATH DOMAIN OF P75 LOW AFFINITY NEUROTROPHIN RECEPTOR, RESIDUES 334-418, NMR, 20 STRUCTURES
NMR structure of the death domain of the p75 neurotrophin receptor.
GNLYSSLPLTKREEVEKLLNGDTWRHLAGELGYQPEHIDSFTHEACPVRALLASWGAQDSATLDALLAALRRIQRADIVE SLCSE
DISTANCE CONSTRAINTS FROM 3D NOESY-15N-HSQC AND 2D NOESY, COUPLING CONSTANTS FROM 3D HNHA-EXPERIMENT, 2QF-COSY, AND 15N-HSQC. A 15N-LABELED SAMPLE OF P75ICD WAS USED. CONSTRAINTS USED: 914 UPPER DISTANCE LIMITS FROM NOE DATA 154 COUPLING CONSTANTS CONSTRAINING DIHEDRAL ANGLES IONIC_STRENGTH: NO SALT ADDED PRESSURE: 1 ATM SOLVENT SYSTEM: 90% H2O/10% D2O
DIANA MINIMIZATION TO TARGET FUNCTION LESS THAN 1, RESTRAINED ENERGY MINIMIZATION USING OPAL
VARIABLE TARGET FUNCTION (DIANA) APPROACH
RECEPTOR, P75, INTRACELLULAR DOMAIN, NEUROTROPHIN RECEPTOR, DEATH DOMAIN
MRRAGAACSAMDRLRLLLLLILGVSSGGAKETCSTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDNVTFSDVVSA TEPCKPCTECLGLQSMSAPCVEADDAVCRCAYGYYQDEETGHCEACSVCEVGSGLVFSCQDKQNTVCEECPEGTYSDEAN HVDPCLPCTVCEDTERQLRECTPWADAECEEIPGRWIPRSTPPEGSDSTAPSTQEPEVPPEQDLVPSTVADMVTTVMGSS QPVVTRGTTDNLIPVYCSILAAVVVGLVAYIAFKRWNSCKQNKQGANSRPVNQTPPPEGEKLHSDSGISVDSQSLHDQQT HTQTASGQALKGDGNLYSSLPLTKREEVEKLLNGDTWRHLAGELGYQPEHIDSFTHEACPVRALLASWGAQDSATLDALL AALRRIQRADIVESLCSESTATSPV