FAMILY OF NMR SOLUTION STRUCTURES OF CA CE CALBINDIN D9K IN DENATURATING CONDITIONS
Monitoring the Early Steps of Unfolding of Dicalcium and Mono-Ce(3+)-Substituted Forms of P43M Calbindin D(9k).
Vitamin D-dependent calcium-binding protein, intestinal
NOESY constraints were obtained on the native form of the protein, while paramagnetic constraints were collected in the presence of 2M Guanidinium Chloride
Uniform labeling with 15N, H2O/D20 90/10
Barbieri R., Bertini I., Cavallaro G., Lee Y.-M., Luchinat C., Rosato A.
L.BANCI,I.BERTINI,M.A.CREMONINI,G.GORI SAVELLINI,C.LUCHINAT,K.WUTHRICH AND P.GUNTERT
CALCIUM BINDING PROTEINS, NMR SOLUTION STRUCTURE, PARAMAGNETISM-BASED CONSTRAINTS, DENATURATING AGENTS, GUANIDINIUM CHLORIDE, METAL BINDING PROTEIN