Solution structure of the ribosome-associated cold shock response protein Yfia of Escherichia coli
Solution structure of the ribosome-associated cold shock response protein Yfia of Escherichia col
A protein residing at the subunit interface of the bacterial ribosome
1H, 13C and 15N resonance assignments of the ribosome-associated cold shock response protein Yfia of Escherichia coli
cold-shock protein Yfia, ribosome associated inhibitor RaiA
MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKEPQGFVADATINTPNGVLVASGKHEDMYTAINELINK LERQLNKLQHKGEARRAATSVKDANFVEEVEEE
Hydrogen bonds were extracted from a series of 15N-HSQC spectra recorded in D2O; Assignment was done using triple-resonance spectra
structures with acceptable covalent geometry, structures with the least restraint violations
Torsion angle dynamics and simulated annealing
0.8mM Yfia, U-15N-13C; 50mM phosphate buffer, 50mM LiCl
0.5mM Yfia, U-15N; 50mM phosphate buffer, 50mM LiCl
0.8mM Yfia; 50mM phosphate buffer, 50mM LiCl
cold shock, translation inhibitor, dsRBD, translation