crystal titration experiments (AMPA co-crystals soaked in 100 nM BrW)

Probing the Function, Conformational Plasticity, and Dimer-Dimer Contacts of the GluR2 Ligand-Binding Core: Studies of 5-Substituted Willardiines and GluR2 S1S2 in the Crystal

PARTIAL AGONIST ACTION DEFINED BY STABILIZATION OF SPECIFIC CONFORMATIONAL SUBSTATES

GLUR-2, GLUR-B, GLUR-K2, GLUTAMATE RECEPTOR IONOTROPIC, AMPA 2

GANKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVY
GKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKGTPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWT
YMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGNAVNLAVLK
LNEQGLLDKLKNKWWYDKGECGS

PEG8000, zinc acetate, sodium cacodylate, pH 6.5 and AMPA, VAPOR DIFFUSION, HANGING DROP, temperature 277K

THE SAME PROTEIN IN COMPLEX WITH BROMO-WILLARDIINE, as a control for crystal titration experiments

THE SAME PROTEIN in complex with AMPA, crystals were then soaked in 10 mM BrW

THE SAME PROTEIN in complex with AMPA, crystals were then soaked in 1 mM BrW

THE SAME PROTEIN in complex with AMPA, crystals were then soaked in 100 uM BrW

THE SAME PROTEIN in complex with AMPA, crystals were then soaked in 10 uM BrW

THE SAME PROTEIN in complex with AMPA, crystals were then soaked in 1 uM BrW

THE SAME PROTEIN in complex with AMPA, crystals were then soaked in 10 nM BrW

THE SAME PROTEIN COMPLEXED WITH AMPA, as a control for crystal titration experiments

THE SAME PROTEIN IN COMPLEX WITH BROMO-WILLARDIINE in a zinc crystal form

IONOTROPIC GLUTAMATE RECEPTOR, GLUR2, LIGAND BINDING CORE, S1S2, PARTIAL AGONIST, Bromo-WILLARDIINE, AMPA, crystal titration, membrane protein

NKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGK
ADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRS
AEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGNAVNLAVLKLNEQ
GLLDKLKNKWWYDKGECGS