TM9A251-284: A Peptide Model of the C-Terminus of a Rat Striated Alpha Tropomyosin
The Structure of the Carboxyl Terminus of Striated alpha-Tropomyosin in Solution Reveals an Unusual Parallel Arrangement of Interacting alpha-Helices
1IHQ IS Glytm1bzip: A Chimeric Peptide Model Of The N-Terminus Of A Rat Short Alpha Tropomyosin With The N-Terminus Encoded By Exon 1b
1TMZ IS Tmzip: A Chimeric Peptide Model Of The N-Terminus Of Alpha Tropomyosin, Nmr, 15 Structures
1IC2 IS Deciphering The Design Of The Tropomyosin Molecule
SEQUENCE THIS SEQUENCE HAS A SYNTHETIC GCG AT N-TERMINUS AND CONTAINS THE MUTATION N279K. THE SEQUENCE DATABASE MATCH, HOWEVER, erroneously HAS A K AT THIS POSITION as well. The sequence in the database should have an ASN at position 279, which has been engineered to LYS in this entry.
THE RESONANCE ASSIGNMENTS WERE MADE USING THROUGH BOND TRIPLE RESONANCE EXPERIMENTS INCLUDING HN(CA)CO, HNCO, H(CA)(C0)NH, H(CA)NH, CA(CO)NH, CANH, CBCA(CO)NH, CBCANH, HCCH-COSY AND CCH-COSY
STRUCTURES WITH COVALENT GEOMETRY, STRUCTURES WITH THE LEAST RESTRAINT VIOLATIONS
REFINEMENT DETAILS CAN BE FOUND IN REFERENCE CITED ABOVE
1648 CONFORMATIONALLY- RESTRICTING NOE CONSTRAINTS (OUT OF A TOTAL OF 2036 NOE DERIVED DISTANCES) 52 LOOSE DIHEDRAL ANGLE CONSTRAINTS AND 48 BACKBONE INTRA- HELICAL HYDROGEN BOND CONSTRAINTS WERE UTILILIZED.
100 MM NACL, 20 MM POTASSIUM PHOSPHATE, 10% DUETERIUM OXIDE, 90% H20, PH 6.4
TROPOMYOSIN, EXON 9A, ACTIN-BINDING, TROPONIN BINDING, MUSCLE, ALPHA-HELIX, COILED-COIL, DIMER, PEPTIDE-MODEL, TWO-CHAINED, DISULFIDE CROSS-LINKED, DE NOVO PROTEIN