Retrovirus envelope domain at 1.7 angstrom resolution.
RESIDUE 94 IS A CYSTEINE IN WILD TYPE P15 PROTEIN. PEPTIDE CONTAINING THIS CYSTEINE CRYSTALLIZES ISOMORPHOUSLY WITH THE ALANINE MUTANT, WAS SOLVED TO 1.8A RESOLUTION (R FACTOR .190 AND HAS A STRUCTURE IS VIRTUALLY IDENTICAL TO THAT OF THE MUTANT. TO GENERATE THE WILD-TYPE MODEL, REPLACE ALA 94 WITH CYSTEINE IN ITS MOST COMMON ROTAMER.
AN AMINO TERMINAL METHIONINE WAS PRESENT AS AN EXPRESSION ARTIFACT. DENSITY FOR THIS METHIONINE WAS NOT VISIBLE IN THE ELECTRON DENSITY. RESIDUE ASP 45 WAS NOT VISIBLE IN THE ELECTRON DENSITY. RESIDUE ASP 98 WAS INCLUDED IN REFINEMENT BUT ITS ATOMS HAVE HIGH B FACTORS AND ITS POSITION SHOULD BE CONSIDERED AMBIGUOUS. THE CHLORIDE ION WAS NOT INCLUDED IN THE REFINEMENT.
GLYCOPROTEIN, COAT PROTEIN, POLYPROTEIN, TRANSMEMBRANE, SIGN, Viral protein
MARSTLSKPLKNKVNPRGPLIPLILLMLRGVSTASPGSSPHQVYNITWEVTNGDRETVWATSGNHPLWTWWPDLTPDLCM LAHHGPSYWGLEYQSPFSSPPGPPCCSGGSSPGCSRDCEEPLTSLTPRCNTAWNRLKLDQTTHKSNEGFYVCPGPHRPRE SKSCGGPDSFYCAYWGCETTGRAYWKPSSSWDFITVNNNLTSDQAVQVCKDNKWCNPLVIRFTDAGRRVTSWTTGHYWGL RLYVSGQDPGLTFGIRLRYQNLGPRVPIGPNPVLADQQPLSKPKPVKSPSVTKPPSGTPLSPTQLPPAGTENRLLNLVDG AYQALNLTSPDKTQECWLCLVAGPPYYEGVAVLGTYSNHTSAPANCSVASQHKLTLSEVTGQGLCIGAVPKTHQALCNTT QTSSRGSYYLVAPTGTMWACSTGLTPCISTTILNLTTDYCVLVELWPRVTYHSPSYVYGLFERSNRHKREPVSLTLALLL GGLTMGGIAAGIGTGTTALMATQQFQQLQAAVQDDLREVEKSISNLEKSLTSLSEVVLQNRRGLDLLFLKEGGLCAALKE ECCFYADHTGLVRDSMAKLRERLNQRQKLFESTQGWFEGLFNRSPWFTTLISTIMGPLIVLLMILLFGPCILNRLVQFVK DRISVVQALVLTQQYHQLKPIEYEP