MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME COMPLEXED WITH LYSOZYME

Three-dimensional structures of the free and the antigen-complexed Fab from monoclonal anti-lysozyme antibody D44.1.

Crystallization and Preliminary X-Ray Diffraction Studies of Two Antigen-Antibody (Lysozyme-Fab) Complexes

DIELTQSPATLSVTPGDSVSLSCRASQSISNNLHWYQQKSHESPRLLIKYVSQSSSGIPSRFSGSGSGTDFTLSINSVET
EDFGMYFCQQSNSWPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVL
NSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

QVQLQESGAEVMKPGASVKISCKATGYTFSTYWIEWVKQRPGHGLEWIGEILPGSGSTYYNEKFKGKATFTADTSSNTAY
MQLSSLTSEDSAVYYCARGDGNYGYWGQGTTLTVSSASTTPPSVFPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSG
SLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPRDC

KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPC
SALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL

VL RESIDUE SER 30 IS IN THE SECOND POSITION OF A II'-TYPE TURN. VL RESIDUE VAL 51 IS THE SECOND RESIDUE (I+1) OF A MODIFIED GAMMA TURN (CLASS 3).

D44.1-HEL COMPLEX CRYSTALS HAVE TWO FAB-HEL COMPLEXES IN THE ASYMMETRIC UNIT. COMPLEX NUMBER 1 INCLUDES CHAIN A (VL AND CL DOMAINS), CHAIN B (VH AND CH1 DOMAINS) AND CHAIN E (HEL). COMPLEX NUMBER 2 INCLUDES CHAIN C (VL AND CL DOMAINS) CHAIN D (VH AND CH1 DOMAINS) AND CHAIN F (HEL). THE CL C-TERMINAL RESIDUE CYS 214 OF COMPLEX NUMBER 1, THE CL C-TERMINAL RESIDUES LYS 213 AND CYS 214 AND CH1 RESIDUES GLY 130 - ASN 136, THR 195, AND VAL 214 HAVE NO ELECTRON DENSITY AND HAVE BEEN ASSIGNED OCCUPANCIES OF 0.01. IN ADDITION, THE 14 C-TERMINAL RESIDUES OF THE HEL OF COMPLEX NUMBER 1 ARE POORLY RESOLVED. ATOMIC POSITIONS FOR ATOMS OF THESE RESIDUES SHOULD BE CONSIDERED ARBITRARY.

FAB D44.1 (IGG1,KAPPA) (BALB/C MOUSE, MONOCLONAL ANTIBODY) COMPLEXED WITH HEN EGG-WHITE LYSOZYME (HEL)

COMPLEX (ANTIBODY-ANTIGEN), COMPLEX (ANTIBODY-ANTIGEN) complex