SOLUTION STRUCTURE OF HUMAN BCL-W PROTEIN
Solution structure of human BCL-w: modulation of ligand binding by the C-terminal helix
ATPASAPDTRALVADFVGYKLRQKGYVCGAGPGEGPAADPLHQAMRAAGDEFETRFRRTFSDLAAQLHVTPGSAQQRFTQ VSDELFQGGPNWGRLVAFFVFGAALCAESVNKEMEVLVGQVQEWMVAYLETRLADWIHSSGGWAEFTALYGDGALEEARR LREGNWASVRLEHHHHHH
The structure was determined using triple-resonance NMR spectroscopy
structures with the least restraint violations,structures with the lowest energy
Structure refined by using standard protocol in CNS with restraints from NOE distances, backbone torsion angles, hydrogen bonds and residual dipolar couplings
1.4 mM Bcl-w protein U-15N,13C, 20 mM phosphate buffer
1.3 mM Bcl-w protein U-15N, 20 mM phosphate buffer
0.5 mM Bcl-w protein U-15N, 6 MG/ML PF1-PHAGE
MATPASAPDTRALVADFVGYKLRQKGYVCGAGPGEGPAADPLHQAMRAAGDEFETRFRRTFSDLAAQLHVTPGSAQQRFT QVSDELFQGGPNWGRLVAFFVFGAALCAESVNKEMEPLVGQVQEWMVAYLETRLADWIHSSGGWAEFTALYGDGALEEAR RLREGNWASVR