Three dimensional solution structure of huwentoxin-IV by 2D 1H-NMR
Function and solution structure of huwentoxin-IV, a potent neuronal tetrodotoxin (TTX)-sensitive sodium channel antagonist from Chinese bird spider Selenocosmia huwena
THIS STRUCTURE WAS DETERMINED USING STANDARD 2D HOMONUCLEAR TECHNIQUES.
THE STRUCTURES ARE BASED ON 527 NOE-DERIVED DISTANCE CONSTRAINTS, 14 DIHEDRAL ANGEL RESTRAINTS AND 9 FAKE DISTANCE RESTRAINTS FROM DISULFIDE BONDS.
5MM HUWENTOXIN-IV MMOL/L PHOSPHATE BUFFER; 90%H2O, 10%D2O
containing a double-stranded beta-sheet and four beta-turns, TOXIN